FIGURE 2 from Identification and Characterization of a Small Molecule Bcl-2 Functional Converter
Year: 2024
Type: other
Abstract: BFC1108 suppresses viability of multiple cancer cell types. A, Bcl-2–selective inhibition of clonogenic survival of MDA-MB-231 cells. MDA-MB-231/Vector and MDA-MB-231/Bcl-2 cells were exposed to 10 µmol/L concentration in medium containing 10% FBS for 48 hours and the colony formation was determined after 2 weeks. B, Quantification of colony formation data shown in A. ***, P < 0.001. C, Bcl-2–dependent induction of apoptosis by BFC1108. MDA-MB-231 cells with high or low Bcl-2 expression were treated with 10 µmol/L BFC1108 in a medium containing 10% FBS for 48 hours and apoptosis was determined by annexin V staining. Data from one representative experiment is shown. Refer to Supplementary Fig. S1 for additional independent experiments. D, BFC1108 inhibits viability of breast cancer cells with Bcl-2 expression. The indicated breast cancer cells were treated for 48 hours, and viability was measured using CellTiter-Glo assay. Data were analyzed by two-way ANOVA and Dunnett multiple comparison post hoc test, **, P < 0.001; ****, P < 0.00001. Bottom panel indicates Bcl-2 RMA-normalized mRNA expression from the Cancer Cell Line Encyclopedia (50). E, BFC1108 suppresses viability of multiple cancer cell types with minimal effects on MCF10A nontransformed breast epithelial cells. Cells were treated for 48 hours in 10% serum containing medium and cell viability was determined. ***, P < 0.001.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4392366730