Imaging of cellular dynamics in vitro and in situ : from a whole organism to sub-cellular imaging with self-driving, multi-scale microscopy
Year: 2024
Type: preprint
Abstract: Many biological processes span multiple time and length scales, including developmental processes and cancer metastasis. While light-sheet fluorescence microscopy (LSFM) has become a fast and efficient method for imaging of organisms, cells and sub-cellular dynamics, simultaneous observations across these scales have remained challenging. Moreover, continuous high-resolution imaging inside living organisms has mostly been limited to few hours as regions of interest quickly move out of view due to sample movement and growth. Here, we present a self-driving, multi-resolution light-sheet microscope platform controlled by a custom Python-based software, to simultaneous observe and quantify sub-cellular dynamics and entire organisms in vitro and in vivo over hours of imaging. We apply the platform to the study of developmental processes, cancer invasion and metastasis, and we provide quantitative multi-scale analysis of immune-cancer cell interactions in zebrafish xenografts.
Institution: The University of Texas Southwestern Medical Center
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Awards: The context-dependent role of Caveolin-1 as a driver of cellular adaptation in Ewing Sarcoma, Next-generation multi-scale microscopy to study metastatic cell extravasation, Transformative microscopes to image across spatiotemporal scales, Imaging mechanisms of metastatic tumor formation in situ
OpenAlex ID: W4392529325