Osteomacs promote maintenance of murine hematopoiesis through megakaryocyte-induced upregulation of Embigin and CD166
Year: 2024
Type: article
Abstract: Maintenance of hematopoietic stem cell (HSC) function in the niche is an orchestrated event. Osteomacs (OM) are key cellular components of the niche. Previously, we documented that osteoblasts, OM, and megakaryocytes interact to promote hematopoiesis. Here, we further characterize OM and identify megakaryocyte-induced mediators that augment the role of OM in the niche. Single-cell mRNA-seq, mass spectrometry, and CyTOF examination of megakaryocyte-stimulated OM suggested that upregulation of CD166 and Embigin on OM augment their hematopoiesis maintenance function. CD166 knockout OM or shRNA-Embigin knockdown OM confirmed that the loss of these molecules significantly reduced the ability of OM to augment the osteoblast-mediated hematopoietic-enhancing activity. Recombinant CD166 and Embigin partially substituted for OM function, characterizing both proteins as critical mediators of OM hematopoietic function. Our data identify Embigin and CD166 as OM-regulated critical components of HSC function in the niche and potential participants in various in vitro manipulations of stem cells.
Source: Stem Cell Reports
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,990
Awards: G1236004326, G1628476732, G1790519008, G4568469465, G4602881204, G4627810106, G4940684865, G5357766052, Osteomacs and megakaryocytes interact to regulate hematopoietic stem cell function, G663624434, G7321284031, CD166 Regulates Human and Murine Hematopoietic Stem Cell Function and the Hematopoietic Niche, BD FACSAria System, G839915751, G9000011061, G9000025480, G9000137147, G9000160688, G9000212862, G9000304140, G998965256
OpenAlex ID: W4392545544