FIGURE 5 from Drug-resilient Cancer Cell Phenotype Is Acquired via Polyploidization Associated with Early Stress Response Coupled to HIF2α Transcriptional Regulation
Year: 2024
Type: other
Abstract: Surviving polyploid cells demonstrate an overall reduction of chromatin openness while AP-1 motifs were enriched. A, Visualization of accessible regions in surviving HCC1806 cells at 0 DPT; as quantified by ATAC-seq. B, Visualization of more (green) or less (red) accessible regions in surviving HCC1806 cells at 0 DPT; as quantified by ATAC-seq. C, Visualization of DNA motifs for AP-1 family members in HCC1806 surviving at 0 DPT, as quantified by ATAC-seq. D, Openness of region for EPAS1 in HCC1806 cells surviving at 0 DPT, as visualized with genome browser tracks. E, Protein level changes in HCC1806 and HCT116 cells of the AP-1 members FOS, FOSL1, JUN, and ATF-3 in untreated (CTL), surviving cells at 0 DPT, 5 DPT, and 10 DPT, and as progeny. F, Number of HCC1806 and HCT116 cells surviving at 0 DPT and 10 DPT when treated with cisplatin alone and cisplatin together with the FOS/AP-1 inhibitor T-5224 from biological replicates (n = 3) and P-value (∗∗, P < 0.01; ∗, P < 0.05, significant relative to vehicle) by ANOVA test as indicated.
Authors: Christopher Carroll, Auraya Manaprasertsak, Arthur Boffelli Castro, Hilda van den Bos, Diana C.J. Spierings, René Wardenaar, Anuraag Bukkuri, Niklas Engström, Etienne Baratchart, Minjun Yang, Andrea Biloglav, Charlie K. Cornwallis, Bertil Johansson, Catharina Hagerling, Marie Arsenian-Henriksson, Kajsa Paulsson, Sarah R. Amend, Sofie Mohlin, Floris Foijer, Alan McIntyre, Kenneth J. Pienta, Emma U. Hammarlund
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4392550680