One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16
Year: 2024
Type: article
Abstract: Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.1
Source: STAR Protocols
Authors: Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Kety Giannetti, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Efthymios Kyrodimos, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios Valakos, Giannis Vatsellas, Russell Petty, Dimitris Thanos, Marco Demaria, Konstantinos Evangelou, Raffaella Di Micco, Vassilis G. Gorgoulis
Institutions: National and Kapodistrian University of Athens, Evangelismos Hospital, Academy of Athens, Biomedical Research Foundation of the Academy of Athens, The San Raffaele Telethon Institute for Gene Therapy, National Hellenic Research Foundation, Pasteur Hellenic Institute, Hippocration General Hospital, University of Crete, FORTH Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, University of Dundee, Ninewells Hospital, University Medical Center Groningen, Manchester Academic Health Science Centre, University of Surrey, University of Manchester
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4392593995