Application of Silica Nanoparticles in Combination with Bacillus velezensis and Bacillus thuringiensis for Anthracnose Disease Control in Shallot
Year: 2024
Type: article
Abstract: Background and Objective: Anthracnose in shallot contributes to significant losses. To solve this issue, silica nanoparticles, in combination with Bacillus velezensis and Bacillus thuringiensis were used together. Materials and Methods: In vitro antagonistic test of Bacillus velezensis B-27 with Colletotrichum gloeosporioides was carried out using dual culture and co-culture methods. Treatment in greenhouse experiments was carried out using single application of silica, B. thuringiensis, B. velezensis, a combination of B. thuringiensis and B. velezensis and a combination of B. thuringiensis, B. velezensis and silica. Detection of B. velezensis in the roots of shallot plants was carried out by PCR using a pair of specific primers. Results: Bacillus velezensis was able to inhibit the growth of C. gloeosporioides mycelium in vitro, both in the dual culture and co-culture methods, by 62.8 and 77.17%, respectively. Treatment of B. thuringiensis and B. velezensis, either individually or in combination with silica, could reduce the intensity of anthracnose disease by 20% each and stimulate the growth of shallot plants. The PCR detection using specific primers on the roots of shallot plants showed that B. velezensis was detected with a DNA band length of ±576 bp. Conclusion: Bacillus velezensis can inhibit the growth of C. gloeosporioides mycelium in vitro. Applying B. velezensis, B. thuringiensis and silica can reduce the intensity of anthracnose disease, promote plant growth and increase plant productivity. Furthermore, B. velezensis was detected in the roots of shallot plants, revealing that the bacteria are well-established.
Authors: Jilan Tsani Abdullah, Suryanti ., Tri Joko
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4392823085