Work

Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner

Year: 2024

Type: article

Abstract: Bioburden detection is crucial for food, water, and biopharmaceutical applications as it can directly impact public health. The objective of this study is to develop and validate an assay and protocol for detecting bioburden on solid surfaces, as well as in water, with high sensitivity and accuracy in a rapid manner. Henceforth, a resazurin‐based assay optimized for detecting bioburden has been integrated with a previously developed portable multichannel fluorometer. The microbes were isolated from solid surfaces in different laboratory settings by swabbing technique, and stream water was collected for contamination analysis. Based on the results, the assay and protocol can successfully detect bioburden as low as 20 CFU/cm 2 and 10 CFU/mL present in both surface and water samples, respectively.

Source: Biotechnology Progress

DOI: 10.1002/btpr.3457

Authors: Md Sadique Hasan, Chad Sundberg, Elias Gilotte, Xudong Ge, Yordan Kostov, Govind Rao

Institution: University of Maryland, Baltimore County

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDGs: Clean water and sanitation, Good health and well-being

Open Access status: green

Funder: U.S. Food and Drug Administration