FIGURE 4 from Activity of the Ubiquitin-activating Enzyme Inhibitor TAK-243 in Adrenocortical Carcinoma Cell Lines, Patient-derived Organoids, and Murine Xenografts
Year: 2024
Type: other
Abstract: TAK-243 inhibits ubiquitylation and induces UPR in ACC cell lines. A, Time course for ubiquitylated proteins and free ubiquitin in cells treated with TAK-243 (500 nmol/L, 2 or 4 hours). The indicated proteins were evaluated by Western blotting. B, Quantitation of the data shown in A. C, Concentration response of ubiquitylated proteins and free ubiquitin in cells treated with the indicated concentrations of TAK-243 for 2 hours. The indicated proteins were evaluated by Western blotting. D, Quantitation of the data shown in B. E, UPR induced by TAK-243 (500 nmol/L, 2 or 4 hours). The samples were from the same cellular lysates as in A, and GAPDH blots were intentionally reused for presentation. The indicated proteins were evaluated by Western blotting.
Authors: Yasuhiro Arakawa, Ukhyun Jo, Suresh Kumar, Nai-Yun Sun, Fathi Elloumi, Anish Thomas, Nitin Roper, Diana Grace Varghese, Naoko Takebe, Xiaohu Zhang, Michele Ceribelli, David O. Holland, Erin Beck, Zina Itkin, Crystal McKnight, Kelli M. Wilson, Jameson Travers, Carleen Klumpp-Thomas, Craig J. Thomas, Chuong D. Hoang, Jonathan M. Hernandez, Jaydira Del Rivero, Yves Pommier
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4392951830