Year: 2024
Type: article
Study design: Observational Study
Abstract: Polyunsaturated fatty acids (PUFAs) generate pro- and anti-inflammatory eicosanoids via three different metabolic pathways. This study profiled tear PUFAs and their metabolites and examined the relationships with dry eye (DE) and meibomian gland dysfunction (MGD) symptoms and signs. A total of 40 individuals with normal eyelids and corneal anatomies were prospectively recruited. The symptoms and signs of DE and MGD were assessed, and tear samples (from the right eye) were analyzed by mass spectrometry. Mann-Whitney U tests assessed differences between medians; Spearman tests assessed correlations between continuous variables; and linear regression models assessed the impact of potential confounders. The median age was 63 years; 95% were male; 30% were White; and 85% were non-Hispanic. The symptoms of DE/MGD were not correlated with tear PUFAs and eicosanoids. DE signs (i.e., tear break-up time (TBUT) and Schirmer's) negatively correlated with anti-inflammatory eicosanoids (11,12-dihydroxyeicosatrienoic acid (11,12 DHET) and 14,15-dihydroxyicosatrienoic acid (14,15, DHET)). Corneal staining positively correlated with the anti-inflammatory PUFA, docosahexaenoic acid (DHA). MGD signs significantly associated with the pro-inflammatory eicosanoid 15-hydroxyeicosatetranoic acid (15-HETE) and DHA. Several relationships remained significant when potential confounders were considered. DE/MGD signs relate more to tear PUFAs and eicosanoids than symptoms. Understanding the impact of PUFA-related metabolic pathways in DE/MGD may provide targets for new therapeutic interventions.
Source: Biomolecules
DOI: 10.3390/biom14030376
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,069
Awards: G1093507213, G113519819, Sphingolipid Metabolism and Signaling in the Retina, G1424293460, G1461384067, G1503134167, Targeted Sphingolipid Metabolism for Treatment of AML, G2034382419, G2595920674, G261043369, G2740587796, G2896096405, G292550038, G3301292218, G3417937016, G3528202847, G3774484590, G3808813408, G3858006751, G4057924396, G4119534169, G4139976473, G4331385445, G4513261217, G4521197144, G458957942, G46069158, AUTOMATED ASSESSMENT OF VISUAL PHOTOSENSITIVITY IN TRAUMATIC BRAIN INJURY. NEW AWARD., G4670433464, G4822413266, G5105958632, G5426736529, G5486522416, G5514656729, G5589565251, G5740957205, G5770983250, G5850682332, G595370322, G6295537351, G646840425, G6560777236, G6584963569, G6732890597, G6810289850, G7520462904, G7522064811, G7680500387, G8173979607, Sphingolipids and their Impact in Corneal Wound Healing, G858300984, GULF WAR ILLNESS: EXPLORING THE EYE-BRAIN CONNECTION, G8693940099, G8957713305, G8987633712, G9000061017, The roles of ceramide and its derivatives in A. phagocytophilum pathogenesis, Defining bacterial members of the ocular surface microbiome and assessing stability over time, University of Virginia Cancer Center Support Grant, Differentiation of Clinical Phenotypes of Inflammatory and Neuropathic Ocular Pain Conditions with Morphologic Measures and Functional Brain Imaging, Miami Center for Vision Research, Tear protein biomarkers of refractive surgery pain, G991732593
OpenAlex ID: W4393003248