Work

Climbing into their Skin to Understand Contextual Protein–Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms

Year: 2024

Type: review

Abstract: Borrowing some quotes from Harper Lee's novel "To Kill A Mockingbird" to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer proteins than biotinylation methods, their ability to probe simultaneously protein function and locale raises new and interesting possibilities for the field.

Source: ChemBioChem

DOI: 10.1002/cbic.202400005

Authors: Marcus J. C. Long, Yimon Aye

Institutions: University of Lausanne, École Polytechnique Fédérale de Lausanne

Language: English

Topic: Biotin and Related Studies

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,220

Funders: École Polytechnique Fédérale de Lausanne, Université de Lausanne