Work

FIGURE 6 from Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Year: 2024

Type: other

Abstract: BMC panel harmony integration for sample-specific batch correction. A, UMAP coordinates generated from concatenated files of 50,000 CD45+, live, singlets from each of 3 BMC donors demonstrate clustering of cells in two-dimensional space (left); however, these data show a strong sample-specific batch effect (middle) and poor cell type-specific clustering (right). B, Following integration using Harmony, new UMAP coordinates are generated (left), the data from 3 donors colocalize (middle), and cells annotated as the same cell type cluster in two-dimensional space (right).

DOI: 10.1158/2767-9764.25471920

Authors: Milos Spasic, Esther R. Ogayo, Adrienne M. Parsons, Elizabeth A. Mittendorf, Peter van Galen, Sandra S. McAllister

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4393144690

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