Work

Figure S1 from Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow

Year: 2024

Type: other

Abstract: Figure S1. PBMC Antibody Staining Performance. (A, B) Histograms showing fluorescence intensity and cell counts when antibodies were used as single-color controls (SC; blue lines) overlaid with staining performance within the full antibody cocktail (multicolor, MC; black lines), in the T/B panel (A) and M/N/D panel (B). The same PBMC donor sample was used for (A and B) and data represent cells gated as singlets, non-RBC, live cells, and the appropriate lymphocyte or monocyte scatter gates. Data were normalized using the time gate to select equal numbers of cells for SC and MC for each marker.

DOI: 10.1158/2767-9764.25471917

Authors: Milos Spasic, Esther R. Ogayo, Adrienne M. Parsons, Elizabeth A. Mittendorf, Peter van Galen, Sandra S. McAllister

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4393157677

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