Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids
Year: 2024
Type: article
Abstract: . Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable 3D architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid development during culture. In this paper, we demonstrate a label-free high-content imaging approach for observing changes in organoid morphology and structural changes occurring at the cellular and subcellular level. Enabled by microfluidic-based culture of 3D cell systems and a novel 3D quantitative phase imaging method, we demonstrate the ability to perform non-destructive high-resolution imaging of the organoid. The highlighted results demonstrated in this paper provide a new approach to performing live, non-destructive monitoring of organoid systems during culture.
Source: Scientific Reports
Authors: Caroline E. Serafini, Seleipiri Charles, Paloma Casteleiro Costa, Weibo Niu, Brian Cheng, Zhexing Wen, Hang Lu, Francisco E. Robles
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,590
Funders: National Science Foundation, U.S. Department of Defense, Burroughs Wellcome Fund, Georgia Institute of Technology, National Institute of Mental Health, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke, Division of Chemical, Bioengineering, Environmental, and Transport Systems, National Science Foundation Graduate Research Fellowship Program
Awards: G270844045, MODELING TSC AND TRANSLATING FOR THERAPEUTICS WITH HUMAN CEREBRAL ORGANOIDS., Graduate Research Fellowship Program (GRFP), G3720455635, CAREER: Understanding the Molecular and Structural Composition of Aggressive Cancers using Deep Ultraviolet Spectroscopy and Imaging, G5811795523, G6671297155, Towards a Better Understanding of Fragile X Syndrome Using Human Brain Organoids, EAGER: Automatic Identification of Writer Accent and Script Influences in Handwriting, Accessible label-free optical microscopy with quantitative molecular and functional contrast, In-line, Non-invasive, Real-time 3D Quantitative Phase Microscopy for Organoids
OpenAlex ID: W4402919886