Supplementary Figure S5 from Development of a [89Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Year: 2024
Type: supplementary-materials
Abstract: Supplementary Figure 5. ELISA shows the affinity of purified human anti-TIP1 full-length IgG1 to TIP1 protein. Recombinant TIP1 protein was coated on ELISA plates. Three-fold serial dilutions (starting at 20 nM) of the purified human anti-TIP1 full-length IgG1 antibodies were incubated with the protein. Anti-human HRP conjugated antibody was used as the detection antibody along with TMB substrate. Absorbance at 450nm vs concentration is plotted in the graph. The data were fitted using the A. “One-site specific” and B. log(agonist) vs. response -- Variable slope (four parameters) model in GraphPad Prism software. The Kd and EC50 values are shown in the table below each graph.
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4393391395