Get started
Work

Supplementary Figure 16 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis

Year: 2024
Type: supplementary-materials
Abstract: Immunoprecipitation of KDM4A-C in WiT49 and HEK293 cells. Reciprocal immunoprecipitation experiments compared to Figure 6 were performed using antibodies against KDM4A, B, C. Elutions of each protein with subsequent western blot for KDM4 A, B, C demonstrate adequate pulldown of KDM4A, B, or C. Immunoprecipitation for KDM4A, B, or C followed by western blot for ubiquitin supports a direct interaction between KDM4A and KDM4C in (A) WiT49 and (B) HEK293 cells. QC6352 may have increased the association between KDM4A and ubiquitin in WiT49 cells.
DOI: 10.1158/1535-7163.25523327.v1
Authors: Prahalathan Pichavaram, Carolyn M. Jablonowski, Jie Fang, Andrew M. Fleming, Hyea Jin Gil, Andrew S. Boghossian, Matthew G. Rees, Melissa M. Ronan, Jennifer A. Roth, Christopher L. Morton, Gerard P. Zambetti, Andrew M. Davidoff, Jun Yang, Andrew J. Murphy
Language: English
Open Access status: gold
OpenAlex ID: W4393438602
Cites:
Cited by:
Related to: 10