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Supplementary Figure 7 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis

Year: 2024
Type: supplementary-materials
Abstract: QC6352 treatment (25nM x 72 hours) reduces nucleoli in WiT49 and HEK293. (A) KDM4A (red) and the nucleolar marker fibrillarin (green) co-localize in the nucleoli [also represented by areas of reduced DAPI staining (blue) in the nucleus due to incompact chromatin in nucleoli] in WiT49 cells. (B) QC6352 treatment caused a reduction in the fibrillarin/nuclear area (determined by the area of fibrillarin/area DAPI) in WiT49 cells (p<0.0001), representing a reduction of nucleoli. (C) Similar findings were found in QC6352-treated HEK293 cells, which also exhibited a (D) reduction of the fibrillarin/nuclear area (p<0.0001).
DOI: 10.1158/1535-7163.25523306
Authors: Prahalathan Pichavaram, Carolyn M. Jablonowski, Jie Fang, Andrew M. Fleming, Hyea Jin Gil, Andrew S. Boghossian, Matthew G. Rees, Melissa M. Ronan, Jennifer A. Roth, Christopher L. Morton, Gerard P. Zambetti, Andrew M. Davidoff, Jun Yang, Andrew J. Murphy
Language: English
Open Access status: gold
OpenAlex ID: W4393498742
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