FIGURE 6 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Year: 2024
Type: other
Abstract: Generation of two-mutation p15E mimotopes that incorporate the 3C mutation. A, Strategy of e-mimotope screening for p15E-3C. (B) Peptide and MHC-I binding prediction by NetMHC algorithms. After vaccination on days 0 and day 7 with the indicated peptides with CPQ, blood was collected on day 14 for wild-type p15E-specific CD8+ T-cell frequency (C) as measured by tetramer staining. On day 14, 1 × 106 MC38 cells were inoculated subcutaneously. Mice received 2 µg peptide of the indicated liposomal vaccine on days 0 and day 7, splenocytes were harvested on day 14 and stimulated with the p15E wild-type peptide for IFNγ production by ELISOPOT (n = 5; D). On day 19 after tumor inoculation, tumor volume (E) was recorded (n = 3). Datapoints represent individual values of 3 mice from the same group in C and E or 5 mice from the same group in D. Statistical analysis applied one-way ANOVA followed by Tukey multiple comparisons tests. *, **, *** and **** indicating P < 0.05, 0.01, 0.001, and 0.0001, respectively. Error bars show mean ± SD.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4393530907