Influenza vaccination stimulates maturation of the human T follicular helper cell response
Year: 2022
Type: dataset
Abstract: Data The two rds-format (R Data Serialization) files contain Seurat objects used for analyses. intergrated_Tcells_harmony_bydonor.rds contains all T cells. intergrated_Tfh_harmony_bydonor.rds is a subset of the previous file containing the TFH and Treg lineages. Set2_10x_clones.tsv is paired TCR information for cells from the 10X experiment for all cells in intergrated_Tcells_harmony_bydonor. TwoYear_LN_Bcell_freq_time.csv contains the B cell subset frequencies in the lymph node by time point. Ali_paired_sc_clones_JCC283.tsv contains paired TCR sequences of cTFH and LN TFH cells obtained by single-cell sorting and target PCR. Ali_bulk_allDonor_clones.tsv contains bulk TCR alpha and beta chains of sorted cTFH and LN TFH generated using a 5'-RACE protocol. The code that accompanies these data is available on GitHub (https://github.com/sschattgen/Flu_TFH_paper) Reads for samples from year 2 for 321-05, year 1 and year 2 312-04, all samples from 321-07, and all samples from 321-08 are available on the NCBI Sequence Read Archive (SRA) under BioProject accession PRJNA1132302. Reads for year 1 samples for 321-05 are available on SRA under BioProject PRJNA610059.
Authors: Stefan Schattgen, Jackson S. Turner, Mohamed A Ghonim, Jeremy Chase Crawford, Aaron J. Schmitz, Hyunjin Kim, Walid Awad, E. Kaitlynn Allen, Mettelman, Robert, Wooseob Kim, Alem Haile, Michael K. Klebert, Teresa Suessen, William D. Middleton, Sharlene A. Teefey, Rachel M. Presti, Ali H. Ellebedy, Paul G. Thomas
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4393559818