The KRASG12C Inhibitor MRTX849 Provides Insight toward Therapeutic Susceptibility of KRAS-Mutant Cancers in Mouse Models and Patients
Year: 2019
Type: dataset
Abstract: We directly interrogated the role of selected genes in mediating therapeutic response to MRTX849 utilizing a focused CRISPR/Cas9 knockout screen targeting approximately 400 genes including many genes involved in KRAS signaling. This was conducted in H358 and H2122 cells in vitro and in H2122 xenografts in vivo in presence and absence of MRTX849 treatment. Three independent drug anchored CRISPR studies were performed and two data files have been provided for each study. The .csv metadata files contain sample IDs, replicate number, sample descriptions and various experimental conditions. The .xlsx data files contain guide labels, guide sequences and NGS guide counts.Three independent drug anchored CRISPR studies were performed and two data files have been provided for each study. The .csv metadata files contain sample IDs, replicate number, sample descriptions and various experimental conditions. The .xlsx data files contain guide labels, guide sequences and NGS guide counts.
Source: Figshare
Authors: Hallin, Jill, Engstrom, Lars D., Hargis, Lauren, Calinisan, Andrew, Aranda, Ruth, Briere, David M., Niranjan Sudhakar, Bowcut, Vickie, Baer, Brian R., Ballard, Josh, Burkard, Michael R., Fell, Jay B., Fischer, John P., Vigers, Guy P., Yaohua Xue, Sole Gatto, Fernandez-Banet, Julio, Pavlicek, Adam, Velastagui, Karen, Chao, Richard C., Barton, Jeremy, Mariaelena Pierobon, Baldelli, Elisa, Petricoin, Emanuel F., Jacobson, Joseph, Marx, Matthew A., Rybkin, Igor I., Johnson, Melissa L., Sai-Hong Ignatius Ou, Lito, Piro, Papadopoulos, Kyriakos P., Jänne, Pasi A., Olson, Peter, Christensen, James G.
Institutions: Mirati Therapeutics (United States), Array BioPharma (United States), Memorial Sloan Kettering Cancer Center, George Mason University, Dana-Farber Cancer Institute, Henry Ford Hospital, Sarah Cannon, Tennessee Oncology, University of California, Irvine, South Texas Accelerated Research Therapeutics
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4393648486