Work

CRISPR-based engineering of RNA viruses

Year: 2023

Type: dataset

Abstract: CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses. This dataset contains code for analyzing sequencing data and generating figures in the manuscript.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.8210144

Authors: Nemudryi, Artem, Nemudraia, Anna, Wiedenheft, Blake

Institution: Montana State University

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Awards: CRISPR-based editing of RNA and cellular mechanisms that govern RNA repair, Structural and functional understanding of bacterial defense and viral counter defense

OpenAlex ID: W4393459405

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Related to: 10