Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment
Year: 2017
Type: dataset
Abstract: Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.
Source: Figshare
Authors: Rocio G. Urdinguio, Lopez, Virginia, Bayón, Gustavo F, Guardia, Rafael Diaz De La, Sierra, Marta I., García-Toraño, Estela, Perez, Raúl F., García, María G., Carella, Antonella, Pruneda, Patricia C., Prieto, Cristina, Dmitrijeva, Marija, Santamarina, Pablo, Thalía Belmonte, Mangas, Cristina, Diaconu, Elena, Ferrero, Cecilia, Tejedor, Juan Ramón, Fernandez-Morera, Juan Luis, Bravo, Cristina, Bueno, Clara, Sanjuan-Pla, Alejandra, Rodríguez, Ramón M., Suarez-Alvarez, Beatriz, López-Larrea, Carlos, Bernal, Teresa, Colado, Enrique, Balbín, Milagros, García-Suarez, Olivia, Chiara, María Dolores, Sáenz-De-Santa-María, Inés, Rodríguez, Francisco, Rodríguez, Francisco, Pando-Sandoval, Ana, Rodrigo, Luis, Santos, Laura, Salas, Ana, Vallejo-Diaz, Jesus, Carrera, Ana Clara, Rico, Daniel, Hernández-López, Inmaculada, Vayá, Amparo, Ricart, José M, Seto, Edward, Sima-Teruel, Núria, Vaquero, Alejandro, Valledor, Luis, Cañal, Maria Jesus, Pisano, David, Graña, Osvaldo, Thomas, Tim, Voss, Anne K., Menéndez, Pablo, Villar-Garea, Ana, Deutzmann, Rainer, Fernandez, Agustín F., Fraga, Mario F.
Institutions: Universidad de Oviedo, Nanomaterials and Nanotechnology Research Center, Hospital Universitario Central de Asturias, Instituto de Investigación Sanitaria del Principado de Asturias, Institució Catalana de Recerca i Estudis Avançats, Josep Carreras Leukaemia Research Institute, Universitat de Barcelona, Consejo Superior de Investigaciones Científicas, Instituto de Investigación Sanitaria La Fe, Instituto Nacional de Silicosis, Centro Nacional de Biotecnología, Newcastle University, Hospital Universitari i Politècnic La Fe, George Washington University, Institut d'Investigació Biomédica de Bellvitge, Spanish National Cancer Research Centre, The University of Melbourne, Walter and Eliza Hall Institute of Medical Research, University of Regensburg
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: European Commission
Award: G371893290
OpenAlex ID: W4393738597