FIGURE 6 from Induction of Viral Mimicry Upon Loss of DHX9 and ADAR1 in Breast Cancer Cells
Year: 2024
Type: other
Abstract: Rescue of PKR activation by DHX9 mutants in SK-BR-3. A, Schematic showing the domain structure of ADAR1 isoforms, DHX9 and mutants of DHX9, dsRBD refers to the dsRNA binding domain. B, Representative immunoblot showing the phenotype of ADAR1 and DHX9 knockdown with DHX9, DHX9K417R or dsRBD-EGFP overexpression in SK-BR-3. Immunoblots for other replicates and uncropped blots can be found in Source Data Figures. C, Fold change of PKR phosphorylation at Thr-446 upon ADAR1 and DHX9 knockdown with DHX9, DHX9K417R or dsRBD-EGFP overexpression in SK-BR-3, quantified from immunoblots in B. Quantification of protein expression for other proteins of interest can be found in Supplementary Fig. S8B–S8F. D, Representative foci formation phenotype of ADAR1 and DHX9 knockdown with DHX9, DHX9K417R or dsRBD-EGFP overexpression in SK-BR-3. Quantification of relative foci area is shown in E. Timepoints for collecting protein lysates and foci formation are the same as described in Fig. 4 for SK-BR-3. Bars represent the average of at least three replicates, error bars are ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P values determined by Dunnett test.
Language: English
Topic: RNA regulation and disease
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4393950409