Work

De novo nanopore sequencing overrepresents RNA modification landscape

Year: 2023

Type: dataset

Abstract: RNA modifications are critical to the functional diversity and regulatory complexity of the transcriptome. With increasing frequency, direct nanopore RNA sequencing is applied to identify RNA modifications de novo. Here, we directly compare the MS2 phage genome RNA modification profiles determined using nanopore to orthogonal LC-MS/MS assays. The results reveal very different views of the modification landscape, suggesting caution when calling new RNA modifications using nanopore alone.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.8403901

Authors: Jones, Joshua D., Goemann, Calvin L.C., Wiegand, Tanner, Nemudryi, Artem, Tardu, Mehmet, Kennedy, Robert T., Wiedenheft, Blake, Koutmou, Kristin S.

Institutions: University of Michigan, Montana State University

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health

Awards: Structural and functional understanding of bacterial defense and viral counter defense, High Resolution Chromatography for Lipids and Proteins, CAREER: Molecular level consequences of mRNA modification, Orbitrap Fusion Lumos ETD with IRMPD for UM Chemistry MS Facility Expansion

OpenAlex ID: W4394022658

Cites:

Cited by:

Related to: 10