Source Data for 'A clinically applicable connectivity signature for glioblastoma includes the tumor network driver CHI3L1'
Year: 2023
Type: dataset
Abstract: Tumor microtubes (TMs) connect glioma cells to a network with considerable relevance for tumor progression and therapy resistance. However, the determination of TM-interconnectivity in individual tumors is challenging and the impact on patient survival unresolved. Here, we establish a connectivity signature from single-cell RNA-sequenced (scRNA-Seq) xenografted primary glioblastoma (GB) cells using a dye uptake methodology, and validate it with recording of cellular calcium epochs and clinical correlations. Astrocyte-like and mesenchymal-like GB cells have the highest connectivity signature scores in scRNA-sequenced patient-derived xenografts and patient samples. In large GB cohorts, TM-network connectivity correlates with the mesenchymal subtype and dismal patient survival. CHI3L1 gene expression serves as a robust molecular marker of connectivity and functionally influences TM networks. The connectivity signature allows insights into brain tumor biology, provides a proof-of-principle that tumor cell TM-connectivity is relevant for patients’ prognosis, and serves as a robust prognostic biomarker.
Source: Figshare
Authors: Hoffmann, Dirk C, Hai, Ling, Wagener, Robin J, D Azorin, Daniel, Hausmann, David, Xie, Ruifan, Huppertz, Magnus-Carsten, Hiblot, Julien, Sievers, Philipp, Heuer, Sophie, Ito, Jakob, Cebulla, Gina, Kourtesakis, Alexandros, Kaulen, Leon D, Ratliff, Miriam, Mandelbaum, Henriette, Jung, Erik, Jabali, Ammar, Horschitz, Sandra, Ernst, Kati, Reibold, Denise, Warnken, Uwe, Venkataramani, Varun, Will, Rainer, Suvà, Mario L, Herold-Mende, Christel, Sahm, Felix, Winkler, Frank, Schlesner, Matthias, Wick, Wolfgang, Kessler, Tobias
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394074651