Additional file 1 of A novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis
Year: 2023
Type: dataset
Abstract: Additional file 1: Fig. S1. Analysis of four candidate circRNAs and enrichment analysis of parent genes. Fig. S2. The mRNA levels of hsa_circ_0052582, hsa_circ_0058113, hsa_circ_0058148, and hsa_circ_0060551 in patients with BC from the GEO dataset (GSE111504). Fig. S3. The mRNA levels of hsa_circ_0052582, hsa_circ_0058113, hsa_circ_0058148, and hsa_circ_0060551 in patients with BC at different T stages from the GEO dataset (GSE111504). Fig. S4. The mRNA levels of hsa_circ_0052582, hsa_circ_0058113, hsa_circ_0058148, and hsa_circ_0060551 in patients with BC at different N stages from the GEO dataset (GSE111504). Fig. S5. CircRNA-miRNA-mRNA ceRNA network. Fig. S6. Expression level of circRRM2/IGF2BP1/MYC in BC and the migration phenotype of miR-27b-3p/miR-31-5p inhibitor in BC cells. Overexpression (A) or knockout (B) efficiency of circRRM2 in BT-549 and MDA-MB-231 was verified by RT qPCR. (C) The levels of IGF2BP1 in BC cells. (D) Expression level of MYC in BC tissues. (E) Correlation analysis of circRRM2 and MYC in BC tissues. (F-G) circRRM2 knockdown abolished the suppression of cell migration treated with miR-27b-3p/miR-31-5p inhibitor. BT-549 cells were transfected with miR-27b-3p (F) or miR-31-5p (G) inhibitor, and the scratch wound healing assay was performed to measure the ability of cell migration. The rescue assay was conducted by co-transfecting the circRRM2 plasmid. *P < 0.05, **P < 0.01, ***P < 0.001. Fig. S7. Prediction and prognostic value of target genes binding with both miR-27b-3p and miR-31-5p. Fig. S8. Prognostic value of target genes binding with both miR-27b-3p and miR-31-5p. *P < 0.05, **P < 0.01, ***P < 0.001. Fig. S9. The transwell assay in BC cell transfected with circRRM2 plasmid and IGF2BP1 siRNA. The transwell assay was performed to detect the rescue effect of overexpression of circRRM2 on IGF2BP1 knockdown in BT-549 (A) or MDA-MB-231 (B) cells. Fig. S10. Calibration plot of the nomogram to predict the probability of the OS in patients with BC at 1, 3, and 5 years. Fig. S11. Analysis of immune cell infiltration related to IGF2BP1 in patients with BC. (A) Lollipop plot shows the correlation between IGF2BP1 level and immunocytes infiltration. (B) Correlations between IGF2BP1 and immune checkpoint-related genes. (C) Scatter plot of correlation between IGF2BP1 and immunocytes infiltration. (D) KM curve of immunocytes infiltration status and OS in patients with BC. (E) Comparison of immunocytes infiltration related with copy number variation of IGF2BP1. *P < 0.05, **P < 0.01, ***P < 0.001. Fig. S12. Mutational analysis of IGF2BP1. (A) The oncoprint of IGF2BP1 was obtained using cBioPortal. (B) Analysis of PTM sites in IGF2BP1. Fig. S13. Network of candidates binding to IGF2BP1 constructed using the BioGRID database. Table S1. Primers used for qRT-PCR. Table S2. The sequences of siRNAs.
Source: Figshare
Authors: Hao, Ran, Zhang, Lei, Si, Yangming, Zhang, Peng, Wang, Yipeng, Li, Bangchao, Hu, Jie, Qi, Yixin
Institutions: Hebei Medical University, Xi'an University of Science and Technology, Tongji University, Liaoning University, Nanjing Agricultural University, Hubei University of Medicine, Shenyang Agricultural University, Central South University, Anhui Agricultural University, East China University of Science and Technology, Army Medical University, Shandong University, Harbin Medical University, Jinan University, China Three Gorges University, Nanjing Forestry University, Zhejiang University of Science and Technology, Pfizer (United States), Shanghai Jiao Tong University, Yunnan University, Jilin University, Anhui Medical University, Chinese Academy of Medical Sciences & Peking Union Medical College, Peking University, Huazhong Agricultural University, Sichuan University, Fudan University, China University of Mining and Technology, North Minzu University, University of Jinan, Sichuan Agricultural University, Soochow University, NYU Langone Health, Sixth Affiliated Hospital of Sun Yat-sen University, Shanghai University of Traditional Chinese Medicine, Third Affiliated Hospital of Harbin Medical University, Foshan Hospital of TCM, Zhongda Hospital Southeast University, Chuzhou University, Research Institute of Forestry, Henan Provincial People's Hospital, Chinese Academy of Agricultural Sciences, The Second Affiliated Hospital of Bengbu Medical College, Second Affiliated Hospital of Anhui Medical University, First Affiliated Hospital of Hebei Medical University, Yellow River Institute of Hydraulic Research, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Xiangya Hospital Central South University, Luoyang Orthopedic-Traumatological Hospital of Henan Province, First Affiliated Hospital of Jinan University, Alfred Health, Inner Mongolia Medical University, Northeast Forestry University, Huazhong University of Science and Technology, Hunan Agricultural University, Xi'an Jiaotong University, Chongqing Medical University, Inner Mongolia University, The University of Texas MD Anderson Cancer Center, Sun Yat-sen University, Chongqing University of Science and Technology, Xuzhou Medical College, Chinese Academy of Sciences, Nantong University, Beijing Normal University, First Affiliated Hospital of Wenzhou Medical University, Chinese PLA General Hospital, Wuhan University, Beijing Tian Tan Hospital, Shandong Tumor Hospital, First Affiliated Hospital of Xi'an Jiaotong University, Ningxia Medical University, First Affiliated Hospital of Shihezi University Medical College, Zhengzhou Fruit Research Institute, Beijing Children’s Hospital, Beijing Friendship Hospital, First Affiliated Hospital of Harbin Medical University, First Affiliated Hospital of Zhengzhou University, The Sixth People's Hospital of Shenyang, Zhejiang Mariculture Research Institute, Children's Hospital of Fudan University, China Astronaut Research and Training Center, Southwest Minzu University, Rockefeller University, Nanjing Normal University, Peking Union Medical College Hospital, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Fourth Hospital of Hebei Medical University, Harvard University, Zhejiang Hospital
Language: English
Topic: Circular RNAs in diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4394074821