Additional file 1: of HE4 promotes collateral resistance to cisplatin and paclitaxel in ovarian cancer cells
Year: 2016
Type: article
Abstract: HE4 overexpression enhances resistance to cisplatin and paclitaxel in OVCAR8 cells. OVCAR8-null vector (NV) and OVCAR8-HE4 clone 5 (C5) cells were treated with 0-500 μM cisplatin for 24 h (A) or 0-5 nM paclitaxel for 48 h (B), at which time the cells were subjected to MTS assay to measure viability. Error bars represent standard deviation of technical replicates. (C) OVCAR8-NV and OVCAR8-C5 cells were treated with vehicle (DMSO) or 100 μM cisplatin, and protein was collected at 24 h after treatment. Western blot was performed to detect levels of PARP and cleaved PARP. (D) Densitometry analysis of PARP and cleaved PARP normalized to β-tubulin levels, from western blot in (C). (PPTX 90 kb)
Source: Figshare
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394105334