Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA
Year: 2023
Type: dataset
Abstract: In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.
Source: Figshare
Authors: Lu, Guili, Wang, Ping, Miao, Shukui, Huang, Jiong, Ma, Wenge, Mi, Xiaoyun, Xue, Jing, Shayilan, Kayizha, Yang, Xueyun, Yan, Genqiang
Institutions: Tongji University, University of Science and Technology of China, Beijing Tongren Hospital, University of Minnesota, Northwell Health, Anhui Agricultural University, Zhongnan University of Economics and Law, Capital Medical University, China Medical University, East Carolina University, Chinese Academy of Medical Sciences & Peking Union Medical College, Ruijin Hospital, Beijing Institute of Genomics, Tianjin Medical University Cancer Institute and Hospital, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell, Affiliated Hospital of North Sichuan Medical College, Affiliated Hospital of Guizhou Medical University, Shanghai Institute of Materia Medica, Jackson Laboratory, Tianjin Children's Hospital, Affiliated Hospital of Qingdao University, First Hospital of Jilin University, Xiyuan Hospital, Chinese Academy of Agricultural Sciences, China Academy of Chinese Medical Sciences, Third Affiliated Hospital of Sun Yat-sen University, Jilin Agricultural University, Beijing Academy of Science and Technology, University of Chinese Academy of Sciences, Henan Agricultural University, Tianjin Medical University, Southern Medical University, Louisiana State University Health Sciences Center New Orleans, Zhejiang University, Lanzhou University, China Medical University, Guangzhou Medical University, Chinese PLA General Hospital, Beijing Tian Tan Hospital, PLA 306 Hospital, Beijing Academy of Agriculture and Forestry Sciences, Second Affiliated Hospital of Zhejiang University, Xiangya Hospital Central South University, Chinese People's Liberation Army, Nanjing University
Language: English
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394179367