ExtFig4A_RACE_D108A_PA_R2-R3_LG384-387_annotated.tif
Year: 2023
Type: dataset
Abstract: HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2, and where indicated, with PR8 PA-X, PR8 PA or a PR8 PA expressing construct with a mutation in the frameshift sequence to prevent PA-X expression (PA(fs)). RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
Source: Figshare
Author: Gaglia, Marta
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4394334397