LncRNA H19 deficiency protects against the structural damage of glomerular endothelium in diabetic nephropathy via Akt/eNOS pathway
Year: 2022
Type: dataset
Abstract: Objective: This study aimed to investigate the functions of lncRNA H19 on glomerular endothelial structural damage of diabetic nephropathy (DN). Materials and Methods: Rats were fed a high sugar and fat high feed die, and intraperitoneally administrated with streptozotocin (30 mg/kg) to induce DN model. Meanwile, rat glomerular endothelial cells (rGEnCs) were treated with high a level of glucose （HG, 30 mM glucose）to induce structural damage. Results: Our results showed that H19 level was drastically increased in diabetic glomeruli and high-glucose (HG)-stimulated rat glomerular endothelial cells (rGEnCs). Deficiency of H19 ameliorated microalbumin, creatinine, BUN, and histopathological alterations in diabetic rats. In addition, H19 deficiency significantly attenuated the damage of endothelial structure by upregulating the expression of junction proteins ZO-1 and Occludin, glycolcalyx protein Syndecan-1, and endothelial activation marker sVCAM-1 and sICAM-1 in diabetic rats. The in vitro results also showed that H19-siRNA alleviated glycocalyx shedding, tight junctions damage, and endothelial activation in HG-stimulated rGEnCs. Moreover, H19 deficiency significantly enhanced the expression of p-Akt and p-eNOS and NO concentration in vitro and in vivo. Pre-treatment with Akt inhibitor LY294002 abrogated these favourable effects mediated by H19 deficiency. Discussion and Conclusion: These results indicate that H19 deficiency could mitigate the structural damage of glomerular endothelium in DN via activating Akt/eNOS pathway.
Source: Figshare
Institutions: Second Military Medical University, Shanghai Jiao Tong University, Capital Medical University, Dalian Medical University, First Affiliated Hospital of Xi'an Jiaotong University, Sichuan Academy of Agricultural Sciences, Harvard University, Wenzhou Medical University, Hubei Polytechnic University, Shanxi Agricultural University, Guangxi University, Nanjing Normal University, Shenzhen University, Chinese Academy of Sciences, China University of Mining and Technology, Zhejiang Ocean University, Sichuan Agricultural University, Max Planck Institute for Demographic Research, Second Affiliated Hospital of Xi'an Jiaotong University, Heilongjiang Academy of Land Reclamation Sciences, Shanghai Academy of Agricultural Sciences, China Agricultural University
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394391180