Jolkinolide B alleviates renal fibrosis via anti-inflammation and inhibition of epithelial-mesenchymal transition in unilateral ureteral obstruction mice
Year: 2021
Type: supplementary-materials
Abstract: Renal fibrosis is a crucial pathological change of chronic kidney disease (CKD) resulting in progressive loss of kidney function. Currently, therapeutic strategies for treating renal fibrosis remain limited. Jolkinolide B (JB) is a natural compound with anti-inflammatory activity from Euphorbia fischeriana Steud. We evaluated effect of JB on kidney fibrosis in mice with unilateral ureteral obstruction (UUO). JB (10 and 30 mg/kg) or vehicle was orally administered once daily for 3 days before occlusion, and then was continued for 10 days. Hematoxylin and eosin (H&E) and Masson stain showed that administration of JB (30 mg/kg) could reduce renal fibrosis and attenuate renal injuries. The results showed that JB reduced the release of inflammatory factors such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, and decreased the expression of extracellular matrix proteins such as fibronectin (FN) and collagen I (Col I). Furthermore, treatment with JB down-regulated the protein levels of phosphorylated nuclear factor-kappaB p65 (p-NF-κB) p65, transforming growth factor-β1 (TGF-β1), p-Smad2/3, α-smooth muscle actin (α-SMA), vimentin and up-regulated the protein level of E-cadherin. The facts confirmed that JB might reduce renal fibrosis and protect the kidneys by inhibiting NF-κB and TGF-β1/Smad pathway coincident with epithelial-mesenchymal transition (EMT).
Source: Figshare
Authors: Li, Mei, Zhang, Wei-Ku, Zhang, Hao-Jun, Yu, Yan, He, Jun, Wang, Yu-Ming, Guo, Yu-Xuan, Wang, Ze-Xing
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394462483