Overlapping functions of RBBP4 and RBBP7 in regulating cell proliferation and histone H3.3 deposition during mouse preimplantation development
Year: 2021
Type: dataset
Abstract: Preimplantation development is critical for reproductive successes in mammals. Thus, it is important to understand how preimplantation embryogenesis is regulated. As a key event of preimplantation development, epigenetic reprogramming has been widely studied, yet how epigenetic complexes regulate preimplantation development remains largely unknown. Retinoblastoma binding protein 4 (RBBP4) and 7 (RBBP7) are integral components of epigenetic complexes including SIN3A, NuRD, and CoREST. Here, we demonstrate that double knockdown of Rbbp4 and 7, but not individually, causes embryonic lethality during the morula-to-blastocyst transition. Mechanistically, depletion of RBBP4 and 7 results in dysregulation of genes related to cell cycle, lineage development, and regulation of transcription, which is accompanied by cell cycle block, disrupted lineage specification and chromatin structure. Interestingly, RBBP4/7 depletion leads to a dramatic increase in H3.3 and H3K27ac abundance during morula-to-blastocyst transition. ChIP-seq analysis in early embryos and embryonic stem cells reveals enrichment of H3.3 at the promoter regions of RBBP4/7 target genes. In summary, our studies demonstrate the compensatory role of RBBP4/7 and reveal its potential mechanisms in preimplantation development. Summary sentence: RBBP4 and RBBP7 play a compensatory role in regulating cell proliferation, apoptosis, and histone H3.3 deposition during preimplantation development.
Source: Figshare
Institutions: Zhejiang University, Guangzhou Center for Disease Control and Prevention, Jilin University, Zhongda Hospital Southeast University, First Hospital of Jilin University, Southeast University, Nanjing Medical University, University of South China, Guangxi University, Sun Yat-sen University, Chongqing University, Chinese Academy of Sciences, Northwest University, Third Affiliated Hospital of Southern Medical University, Key Laboratory of Guangdong Province, Xi'an Honghui Hospital, Hebei General Hospital, First Affiliated Hospital of Xi'an Jiaotong University, Yunnan Academy of Agricultural Sciences, National Institutes for Food and Drug Control, Tianjin Medical University, Henan Normal University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4394506128