This work has been retracted.
RETRACTED ARTICLE: Recombinant human klotho protects against hydrogen peroxide-mediated injury in human retinal pigment epithelial cells via the PI3K/Akt-Nrf2/HO-1 signaling pathway
Year: 2022
Type: dataset
Abstract: Globally, age-related macular degeneration (AMD) is a common irreversible ophthalmopathy. Oxidative stress of retinal pigment epithelial cells is involved in AMD occurrence and development. Klotho is an anti-aging protein with antioxidant properties. We investigated the protective properties of Klotho on hydrogen peroxide (H2O2)-induced injury of retinal pigment epithelial cells (ARPE-19 cells) and its associated pathomechanisms. We found that Klotho pretreatment for 24 h could up-regulate Bcl-2 levels, decrease the cleaved-caspase-3 and Bax levels, inhibit H2O2-induced ARPE-19 cell apoptosis, and promote cell proliferation. Klotho pretreatment inhibited the H2O2-mediated elevations of reactive oxygen species (ROS) in ARPE-19 cells. It enhanced antioxidant activities of the cells and restored the glutathione peroxidase (GPX), superoxide dismutase (SOD2), catalase (CAT), as well as malondialdehyde (MDA) levels to close to the normal level. N-acetylcysteine (NAC), a reactive oxygen scavenger, could reverse the harmful effects of H2O2 on proliferation, apoptosis, and oxidative stress of ARPE-19 cells. Further, Klotho pretreatment enhanced Akt phosphorylation and expression as well as nuclear translocation of Nrf2 in H2O2-treated ARPE-19 cells. This indicates that Klotho protects cells from oxidative stress by activating phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)-nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) signaling pathway. Klotho is, therefore, a potential preventive or treatment option for AMD.
Source: Figshare
Institutions: South China Agricultural University, Shenzhen Science and Technology Innovation Commission, Tongji University, Central South University, Shanghai Jiao Tong University, Second Xiangya Hospital of Central South University, The University of Texas Health Science Center at Houston, Air Force Medical University, Stanford University, Shihezi University, Fujian Medical University, Nanjing University of Chinese Medicine, Shandong University, Sun Yat-sen University, Jinan University, Xuzhou Medical College, Yangtze University, Capital Medical University, Yunnan University, Chinese Academy of Medical Sciences & Peking Union Medical College, Washington University in St. Louis, Sichuan University, Fudan University, Renji Hospital, Southern University of Science and Technology, Sichuan Agricultural University, Northwest University, Soochow University, Guangzhou Center for Disease Control and Prevention, National Institute of Neurological Disorders and Stroke, The First Affiliated Hospital, Sun Yat-sen University, Peking University International Hospital, Chinese Academy of Agricultural Sciences, Xijing Hospital, China Agricultural University, Case Western Reserve University, University of Duisburg-Essen, Zhejiang University, Nanjing Medical University
Language: English
Subfield: Ophthalmology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4394512346