FIGURE 1 from Inhibition of NEK2 Promotes Chemosensitivity and Reduces KSHV-positive Primary Effusion Lymphoma Burden
Year: 2024
Type: other
Abstract: NEK2 depletion decreases PEL survival. A, Western blot analysis for NEK2 expression in PEL cells. B, Western blot analysis for NEK2 expression in normal PBMCs alongside PEL cells. C–D, Western blots for shRNA-mediated knockdown of NEK2 expression in PEL cells and subsequent cell viability assays measuring PEL survival over time after treatment with two different NEK2 shRNAs (dotted lines) or nontemplate control (NTC, solid black line). Data are representative of three independent biological replicates, each performed in triplicate. Viability data were normalized to 0-hour luminescence values for each treatment, fitted using nonlinear regression, and analyzed using two-way ANOVA with Dunnett multiple comparisons. ****, P < 0.0001. C, ***P = 0.0001; **, P < 0.0005. D, ***, P < 0.0005; *, P = 0.0009, E, ***, P = 0.0003. The BCBL1 loading control blot in C is the same as in Fig. 3E.
Authors: Maria C. White, Jason P. Wong, Blossom Damania
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4394602508