Work

An optimized protocol for generating appendage-bearing skin organoids from human-induced pluripotent stem cells

Year: 2024

Type: article

Abstract: Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs in vitro, as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.g. hair follicles, sebaceous glands, etc.) across hiPSC lines from two different somatic sources.

Source: Biology Methods and Protocols

DOI: 10.1093/biomethods/bpae019

Authors: Imaan Ahmed, Jane Sun, Jason Brown, Kiarash Khosrotehrani, Abbas Shafiee

Institutions: The University of Queensland, Queensland Health, Royal Brisbane and Women's Hospital, Metro North Hospital and Health Service

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,198