Work

Purification of Native Dentilisin Complex from Treponema denticola by Preparative Continuous Polyacrylamide Gel Electrophoresis and Functional Analysis by Gelatin Zymography

Year: 2024

Type: article

Abstract: . • The protocol involves large-scale preparative electrophoresis using a Model 491 Prep Cell. • We then use gelatin zymography to demonstrate the activity of the purified dentilisin complex by its ability to activate matrix metalloproteinase 2 (MMP-2).

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.4970

Authors: Pachiyappan Kamarajan, John Timm, M. Paula Goetting-Minesky, Erin Malone, Sean Ganther, Allan Radaic, Christian Tafolla, J. Fenno, Yvonne Kapila

Institutions: University of California, Los Angeles, University of Michigan, University of California, San Francisco

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Institutes of Health, National Institute of Dental and Craniofacial Research

Awards: Surface protein complexes of oral treponemes: assembly and host cell interactions, Characterization of Treponema Denticola-Mediated Interactions With Periodontal Ligament Cells Leading to Persistent Host Cell/Tissue Destruction, Treponema - Host Cell and Tissue Interactions, Oral and Craniofacial Sciences Health Research Training

OpenAlex ID: W4394820196

FWCI: 0.914

Cites: 40

Cited by: 2

Related to: 10