Work

Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities

Year: 2024

Type: review

Abstract: Metabolic flux analysis (MFA) is a valuable tool for quantifying cellular phenotypes and to guide plant metabolic engineering. By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical pathways. Recent applications of isotopically nonstationary MFA (INST-MFA) to plants have elucidated nonintuitive metabolism in leaves under optimal and stress conditions, described coupled fluxes for fast-growing algae, and produced a synergistic multi-organ flux map that is a first in MFA for any biological system. These insights could not be elucidated through other approaches and show the potential of INST-MFA to correct an oversimplified understanding of plant metabolism.

Source: New Phytologist

DOI: 10.1111/nph.19734

Authors: Somnath Koley, Poonam Jyoti, Maneesh Lingwan, Doug K. Allen

Institutions: Donald Danforth Plant Science Center, Agricultural Research Service, United States Department of Agriculture

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green