Year: 2024
Type: preprint
Abstract: In this section, generate nuclear coordinates on thehigh-resolution H&E images through color deconvolution and identificationof two-dimensional intensity minimums in the hematoxylin channels of the images(corresponding to the dark blue nuclei). This calculation is done on thehigh-resolution tiff images saved inside pth10x. Note on cell detection: It is important that the cellcoordinates are generated on unregistered images, as warping caused by theregisteration process can cause inaccuracies in nuclear detection performed onthe registered images. Instead, detect coordinates on the unregistered images,then apply the registration transformations to the cell coordinates todetermine their positions in registered space.
Institutions: Johns Hopkins University, Johns Hopkins Medicine
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Funder: National Cancer Institute
Award: G601512365
OpenAlex ID: W4394934286