Figure S3 from Epithelial Expressed B7-H4 Drives Differential Immunotherapy Response in Murine and Human Breast Cancer
Year: 2024
Type: other
Abstract: Supplemental Figure 3. B7-H4 was expressed on some tissue immune cells in the C57BL/6 model, but not the BALB/c model. (A) Formalin-fixed, paraffin-embedded sections of BALB/c and C57BL/6 mice were stained for B7-H4, CD45, and DAPI using multiplexed immunofluorescence, or B7-H4 by IHC. CD45+ B7-H4+ cells were observed in spleen and intestine in C57BL/6 mice but not BALB/c mice. (B) Based on morphological characterization, these are likely macrophages. BALB/c spleen, intestine, and other healthy tissues examined (lymph node, fat pad, lung) had no B7-H4+ immune cells. Scale bar 20µm. (C) Flow cytometry of B7-H4+ cells are not present in the BALB/c spleen but are in the C57BL/6 spleen, similar to our findings by mIF. (D) Representation of flow cytometry scatter plots. B7-H4+ immune cells were only found in the C57BL/6 spleen, not bone marrow (b.m.)
Authors: Elizabeth C. Wescott, Xiaopeng Sun, Paula Gonzalez-Ericsson, Ann Hanna, Brandie C. Taylor, Violeta Sanchez, Juliana Bronzini, Susan R. Opalenik, Melinda E. Sanders, Julia Wulfkuhle, Rosa I. Gallagher, Henry Gomez, Claudine Isaacs, Vijaya Bharti, John T. Wilson, Tarah J. Ballinger, Cesar A. Santa-Maria, Payal D. Shah, Elizabeth C. Dees, Brian D. Lehmann, Vandana G. Abramson, Gillian L. Hirst, Lamorna Brown Swigart, Laura J. van ˈt Veer, Laura J. Esserman, Emanuel F. Petricoin, Jennifer A. Pietenpol, Justin M. Balko
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4395082293