Year: 2024
Type: letter
Abstract: In their ‘Matters Arising’ manuscript, Saurabh et al. discuss two issues related to single-molecule Förster resonance energy transfer (smFRET) experiments: the use of the Gaussian noise approximation and spectral crosstalk. Their arguments are based on simulations obtained with parameters that differ significantly from the typical conditions measured experimentally, and, thus, from the regime included in the original study (Götz et al. 1 ). In addition, they make claims about our multi-lab blind study that we would like to rectify. In Table 1 , we provide a list of specific statements made by Saurabh et al. with our respective explanations. Table 1 Specific statements by Saurabh et al. with respective explanations Full size table
Source: Nature Communications
Authors: Markus Götz, Anders Barth, Søren S. -R. Bohr, Richard Börner, Jixin Chen, Thorben Cordes, Dorothy A. Erie, Christian Gebhardt, Mélodie C. A. S. Hadzic, George L. Hamilton, Nikos S. Hatzakis, Thorsten Hugel, Lydia Kisley, Don C. Lamb, Carlos de Lannoy, Chelsea Mahn, Dushani Dunukara, Dick de Ridder, Hugo Sanabria, Julia Schimpf, Claus A. M. Seidel, Roland K. O. Sigel, Magnus B. Sletfjerding, Johannes Thomsen, Leonie Vollmar, Simon Wanninger, Keith R. Weninger, Pengning Xu, Sonja Schmid
Institutions: PicoQuant (Germany), Delft University of Technology, University of Copenhagen, Novo Nordisk Foundation, Hochschule Mittweida, University of Zurich, Ohio University, Ludwig-Maximilians-Universität München, University of North Carolina at Chapel Hill, UNC Lineberger Comprehensive Cancer Center, New York University, Clemson University, University of Freiburg, Case Western Reserve University, Center for NanoScience, Wageningen University & Research, North Carolina State University, Heinrich Heine University Düsseldorf, University of Basel
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,790
OpenAlex ID: W4396519595