FIGURE 3 from Small Extracellular Vesicles Promote Stiffness-mediated Metastasis
Year: 2024
Type: other
Abstract: Stiff EVs show increased biodistribution and retention in vivo.A, Schematic of EV isolation and staining prior to tail-vein injection of 10 µg of EVs in nude mice. NIR imaging (B) and mean SNR (C) of MDA-MB-231 vesicle biodistribution in dorsal, left, ventral, and right sides (mean ± SEM). Signal intensity is in arbitrary units (a.u.). Three mice in 0.5 kPa condition, and four in 25 kPa condition; one-way ANOVA. NIR imaging (D) and mean SNR biodistribution (E) in the lungs, liver, spleen, kidneys, and bone/bone marrow (mean ± SEM). Signal intensity is in arbitrary units (a.u.). Three mice in MDA-MB-231 0.5 kPa condition and four in 25 kPa condition; one-way ANOVA. F, MDA-MB-231 EV binding assay to ECM proteins collagen type I, collagen type IV, and laminin (mean ± SEM). Three biological repeats for collagen type I and collagen type IV; five for laminin. Paired t test.
Authors: Alexandra Sneider, Ying Liu, Bartholomew Starich, Wenxuan Du, Praful R. Nair, Carolyn Marar, Najwa Faqih, Gabrielle E. Ciotti, Joo Ho Kim, Sejal Krishnan, Salma Ibrahim, Muna Igboko, Alexus Locke, Daniel M. Lewis, Hanna Hong, Michelle N. Karl, Raghav Vij, Gabriella C. Russo, Estibaliz Gómez-de-Mariscal, Mehran Habibi, Arrate Muñoz-Barrutia, Luo Gu, T.S. Karin Eisinger-Mathason, Denis Wirtz
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4396769972