Work

Reviewer #3 (Public Review): ESAT-6 undergoes self-association at phagosomal pH and an ESAT-6 specific nanobody restricts M. tuberculosis growth in macrophages

Year: 2024

Type: peer-review

Abstract: eLife assessmentThis useful study investigates two secreted Mycobacterium tuberculosis proteins, ESAT-6 and CFP10, using biochemical assays, including a Biolayer Interferometry assay.Solid experimental evidence demonstrates that ESAT-6 forms a tight interaction with CFP10 as a heterodimer at neutral pH and that ESAT-6 also forms a homodimer at acidic pH.Additional, more definitive evidence is required to describe how these proteins disrupt the phagosomal membrane.While improved compared to a previous version, the revised manuscript did not address these concerns adequately.

Source: eLife

DOI: 10.7554/elife.91930.2.sa0

Language: English

Topic: Tuberculosis Research and Epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,500

Funders: Bill and Melinda Gates Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases

Awards: G6322174463, Determining the role of sphingolipids in Mycobacterium tuberculosis infection, Multidisciplinary Research Training in Pulmonary Medicine