Hardware and software solutions for implementing nanospray desorption electrospray ionization (nano‐DESI) sources on commercial mass spectrometers
Year: 2024
Type: article
Abstract: Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization mass spectrometry imaging (MSI) approach that enables spatial mapping of biological and environmental samples with high spatial resolution and throughput. Because nano-DESI has not yet been commercialized, researchers develop their own sources and interface them with different commercial mass spectrometers. Previously, several protocols focusing on the fabrication of nano-DESI probes have been reported. In this tutorial, we discuss different hardware requirements for coupling the nano-DESI source to commercial mass spectrometers, such as the safety interlock, inlet extension, and contact closure. In addition, we describe the structure of our custom software for controlling the nano-DESI MSI platform and provide detailed instructions for its usage. With this tutorial, interested researchers should be able to implement nano-DESI experiments in their labs.
Source: Journal of Mass Spectrometry
DOI: 10.1002/jms.5065
Authors: Li‐Xue Jiang, Ryan T. Hilger, Julia Laskin
Institution: Purdue University West Lafayette
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,220
Awards: G1150665490, Development of a high throughput system for molecular imaging of different cell types in mouse brain tissues, G3110643040, Novel Platform for Quantitative Subcellular Resolution Imaging of Human Tissues Using Mass Spectrometry, Pushing the Frontiers of Identification and Coverage in High-Resolution Spatially-Resolved Lipidomics
OpenAlex ID: W4399655559