Work

An engineered T7 RNA polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis

Year: 2024

Type: article

Abstract: We have engineered a novel RNA polymerase, T7-68, that improves co-transcriptional incorporation of selected cap analogs and reduces dsRNA content, addressing manufacturing challenges and facilitating large-scale mRNA production.

Source: Faraday Discussions

DOI: 10.1039/d4fd00023d

Authors: Mathew Miller, Oscar Alvizo, Scott Baskerville, Avinash Chintala, Chinping Chng, Justin Dassie, Jonathan Dorigatti, Gjalt Huisman, Stephan Jenne, Supriya Kadam, Neil Leatherbury, Stefan Lutz, Melissa Mayo, Arpan Mukherjee, Antoinette Sero, Stuart Sundseth, Jonathan Penfield, James Riggins, Xiyun Zhang

Institutions: Codexis (United States), Precision BioSciences (United States)

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W4396945872

FWCI: 4.445

Cites: 43

Cited by: 44

Related to: 10