Spatial Profiling of Metals through Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging
Year: 2024
Type: preprint
Abstract: The spatial-omic analysis of biomolecules such as nucleic acids, lipids, metabolites, and proteins is advancing the study of biological systems and processes in a physio-pathological context. Here, we describe an innovative matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) method to detect metals within biological tissues using instrumentation that is widely available in research and clinical laboratories. We characterize the spatial distribution of metals in diverse settings including mouse embryogenesis, genetic disorders leading to abnormal metal accumulation, and preclinical testing for improved platinum-based chemotherapy delivery through focused ultrasound across the blood-brain barrier. Spatial metal profiling will advance research studies and the clinical analysis of metal-related diseases, enabling more precise use of metal-based therapies and advances in diverse scientific fields beyond biomedicine. One-Sentence Summary Spatial metallomic profiling maps native metals or those coordinated to xenobiotics, antibodies, and biomolecules in tissues.
Authors: Sylwia A. Stopka, Clément Bodineau, Gerard Baquer, Jia-Ren Lin, Md Amin Hossain, Michael S. Regan, Daniela Ruiz, Stecia-Marie Fletcher, Olivier Pourquié, Svetlana Lutsenko, Connor N. Payne, Jeffrey N. Agar, Ralph Mazitschek, Nathan J. McDannold, Peter K. Sorger, Sandro Santagata, Nathalie Y. R. Agar
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Pre-cancer atlases of cutaneous and hematologic origin (PATCH Center), Systems Pharmacology of Therapeutic and Adverse Responses to ImmuneCheckpoint and Small Molecule Drugs, Center of Innovation for Brain Tumor Therapeutics, Advancing technologies for the collection and analysis of high dimensional immunoprofiles and tumor images, Harvard/Stanford GTN Program: Novel targeted therapeutics for glioblastoma
OpenAlex ID: W4398233421