Work

Synergism of Endo and Exo-α-1,3-Glucanases in α-1,3-Glucan Degradation: A Kinetic Study

Year: 2024

Type: article

Abstract: Bio-based materials are promising alternatives to traditional fossil derived synthetic polymers, to mitigate green house gas emissions and to provide end-of-life benefits addressing increasing environmental concerns. The degradation and reuse of biobased polymers attract increasing attention. In this work, a dual-enzyme system (combining endo α-1,3-glucanase Agl-ST from Streptomyces thermodiastaticus HF 3-3 and exo α-1,3-glucanase YgjK from Escherichia coli K12) was identified for the targeted degradation of α-1,3-glucan. The effect of pH, metal ions, enzyme concentration, temperature, and reaction time were investigated. After degradation under model conditions for 10 h, the dual-enzyme system achieved a weight loss rate of 29%, releasing 4.0 mM reducing sugar from 1% α-1,3-glucan. Binding behavior and degradation kinetics of α-1,3-glucanase on α-1,3-glucan were studied by quartz crystal microbalance with dissipation monitoring (QCM-D). This dual-α-1,3-glucanase enzyme cocktail is a promising example for efficient bio-based α-1,3-glucan polymer degradation, thereby contributing towards the concept of a circular economy.

Source: ACS Sustainable Chemistry & Engineering

DOI: 10.1021/acssuschemeng.4c01469

Authors: Zhe Dong, Peng Zhang, Slavko Kralj, Yu Ji, Ulrich Schwaneberg

Institutions: RWTH Aachen University, International Flavors & Fragrances (United States)

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: green

Funder: Bundesministerium für Bildung und Forschung