Work

A continuous-wave spectroscopic singlet oxygen sensor for photodynamic therapy dosimetry: Preclinical studies in a murine model of actinic keratosis

Year: 2024

Type: article

Abstract: Singlet oxygen (1O2), a type of reactive oxygen species, destroys cancer cells during photodynamic therapy (PDT). Inter-patient variations in tissue oxygen levels, dose of photosensitizer (PS), and fluence of light can affect 1O2 generation and result in suboptimal treatment efficacy in the clinic. Here, we describe a high throughput spectrometer system based on computational spectroscopy for real-time measurement of PS levels and 1O2 luminescence during PDT. The dosimeter provides a direct real-time measurement of 1O2 production in continuous-wave mode. Data were obtained from in-vitro protoporphyrin IX (PpIX) phantoms, and from in-vivo studies in mice with actinic keratosis (AK, an early form of squamous cell carcinoma) using the dosimeter. Data indicated a correlation between 1O2 levels and treatment outcome (lesion shrinkage/clearance). This study provides additional evidence for a correlation between 1O2 generation and AK treatment response, suggesting that 1O2 dosimetry could have utility in the dermatology clinic.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2024.104125

Authors: Sanjay Anand, Tiffany Yu, Cheng-En Cheng, Steven J. Davis, Youbo Zhao, Edward V. Maytin

Institutions: Cleveland Clinic Lerner College of Medicine, Physical Sciences (United States)

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,450