Supplementary Figure S1 from Repolarization of Immunosuppressive Macrophages by Targeting SLAMF7-Regulated CCL2 Signaling Sensitizes Hepatocellular Carcinoma to Immunotherapy
Year: 2024
Type: supplementary-materials
Abstract: (A) The mRNA expression of SLAMF7 between peri-tumor (PT) and tumor (TT) tissues of GSE14520 cohort (n=247). (B) qPCR and immunoblot assays of SLAMF7 expression in hepatic cell line L-02 and HCC cell lines. (C) qPCR and immunoblot assays verifying the knockdown and overexpression efficiency of SLAMF7 in HCC cells. (D-E) CCK8 and migration assays showing the effect of SLAMF7 on the proliferative and migration abilities of HCC cells. (F-G) Left: Gross appearance of the liver bearing orthotopic Hepa1-6 tumors with and without Slamf7 overexpression from C57BL/6 and NOG mice. Right: The tumor weight and tumor volume of each group (5 mice per group). (H-I) Representative H&E staining images of lungs (left) and the incidence of lung metastasis (right) in the indicated orthotopic HCC mouse models from C57BL/6 and NOG mice. Scale bar: 500 μm (top) and 100 μm (bottom). ***P<0.001, and NS, not significant. Student’s t test (or one-way ANOVA with a post hoc LSD test in part A). Experiments were repeated three time and results were present as the mean ± SD.
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4399334367