Supplementary Figure S8 from Structural Basis for Multivalent MUC16 Recognition and Robust Anti-Pancreatic Cancer Activity of Humanized Antibody AR9.6
Year: 2024
Type: supplementary-materials
Abstract: Figure S8. huAR9.6 induces ADCC and CDC in PDAC cells. (A) Flow cytometry-based assay showing ADCC against CFSE-labeled T3M4, Capan-2 and T3M4-MUC16KO cells via FVD (viability stain) CFSE double positive cells following incubation with peripheral blood mononuclear cells (PBMCs, effector cells) with or without the huAR9.6 antibody (B) Flow cytometry-based assay showing CDC against target T3M4, Capan-2 and T3M4-MUC16KO cells using Annexin-V/PI staining following incubation with human healthy donor derived serum with or without the huAR9.6 antibody.
Authors: Eric N. Aguilar, Satish Sagar, Brandy R. Murray, Christabelle Rajesh, Eric K. Lei, Sarah A. Michaud, David R. Goodlett, Thomas C. Caffrey, Paul M. Grandgenett, Benjamin Swanson, Teresa M. Brooks, Adrian R. Black, Henk van Faassen, Greg Hussack, Kevin A. Henry, Michael A. Hollingsworth, Cory L. Brooks, Prakash Radhakrishnan
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4399342809