Work

Split intein mediated protein polymerization for microbial production of materials

Year: 2024

Type: article

Abstract: The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.

Source: OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Author: Zhang, Fuzhong; Bowen, Christopher H.; Dai, Bin

Language: English

Topic: Biochemical and Structural Characterization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4399455271

FWCI:

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