FIGURE 2 from Hemangiosarcoma Cells Promote Conserved Host-derived Hematopoietic Expansion
Year: 2024
Type: other
Abstract: Hematopoietic expansion derived from adoptive transplantation of canine hemangiosarcoma in immunodeficient mice. A and B, Xenotransplantation of canine hemangiosarcoma cell lines created exuberant myeloid hyperplasia in mouse spleens. Representative photomicrographs show histopathology by H&E staining of spleens from NSG mice transplanted with hemangiosarcoma cell lines SB (A) and EFB (B). C, Immunoreactivity of anti-mouse Ki-67 (Tec-3) antibody shows strongly positive signal in proliferating cells in the spleen. D, Immunostaining of anti-human (and canine cross-reactive) Ki-67 (MiB-1) antibody shows lack of positive staining among proliferating cells. E, The proliferating cells are immunoreactive with anti-mouse Ter-119 antibody. F, SB cells expressing Luciferase are detected in mouse spleen (arrows). Images shown in C, D, E, and F are from IHC staining done in mice inoculated with SB cells modified to express GFP and firefly Luciferase. Horseradish peroxidase (for Ki-67 stains) and alkaline phosphatase (for Ter-119 and Luciferase) conjugates were used. Counterstain = hematoxylin. A–D: Bar = 200 µm; E–F: Bar = 50 µm.
Authors: Jong Hyuk Kim, Ashley J. Schulte, Aaron L. Sarver, Donghee Lee, Mathew G. Angelos, Aric M. Frantz, Colleen L. Forster, Timothy D. O'Brien, Ingrid Cornax, M. Gerard O'Sullivan, Nuojin Cheng, Mitzi Lewellen, LeAnn Oseth, Sunil Kumar, Susan Bullman, Chandra Sekhar Pedamallu, Sagar M. Goyal, Matthew Meyerson, Troy C. Lund, Matthew Breen, Kerstin Lindblad-Toh, Erin B. Dickerson, Dan S. Kaufman, Jaime F. Modiano
Language: English
Topic: Veterinary Oncology Research
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4399528215